Effect of low-intensity eccentric resistance training with blood flow restriction on the activation and proliferation indicators of satellite cells

Introduction: The objective of the present research was to determine the effect of low-intensity eccentric training with blood flow restriction (BFR) on Hepatocyte Growth Factor (HGF) and myogenic factor 5 (Myf5), in non-athletes young men. Methods: In this study, 20 non-athlete young men were allocated to two eccentric training and eccentric training with BFR groups. Eccentric training program includes 75 knee extension repetitions in severity of 30% IRM within three sets by using isokinetic device. BFR was made by using air pressure meter and a muscular biopsy was taken from the vastus lateralis muscle of active leg, 48 hours before and 24 hours after the training program. The data were analyzed using independent and paired t-tests. Results: The results of analysis showed that HGF serum levels and mRNA Myf5 expression in both eccentric resistance training and without BFR groups had a significant increase compared to pre-test levels (P≤0.05). Moreover, it was observed that HGF serum levels and mRNA Myf5 expression in training group with BFR had a significant increase of 6.39% and 4.05%, respectively, compared to training group without BFR (P≤0.05). Conclusion: The results of this research showed that eccentric training with and without BFR may improve the activation and proliferation indicators of satellite cells. But, in spite of this matter, it seems that eccentric resistance training with BFR may have a more significant effect on activation and reproduction of satellite cells.